NUCLEOSIL
NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
- Wide acceptance as routine packings for very different fields of chromatography.
- One of the first spherical silicas used in HPLC.
- An absolutely reliable choice in HPLC.
- The largest variety of modified HPLC silicas available on the market.
Summary Of Available NUCLEOSIL Phases
| Phase | Modification | % C | pH Range | Particle Sizes [µm] | USP Classification |
| RP phases | |||||
| NUCLEOSIL® C18 | medium density octadecyl, endcapped | 15 | 2-8 | 3,5,7,10 µm | L1 |
| NUCLEOSIL® C18 AB | crosslinkedoctadecyl, endcapped, high steric selectivity | 25 | 1-9 | 5 µm | L1 |
| NUCLEOSIL® C18 HD | high density octadecyl, endcapped | 20 | 2-9 | 3,5 µm | L1 |
| NUCLEOSIL® SiOH | Unmodified | – | 2-8 | 5 µm | L3 |
| NUCLEOSIL® C8 ec | medium density octyl, endcapped | 8.5 | 2-8 | 5 µm | L7 |
| NUCLEOSIL® C8 | standard octyl, not endcapped | 8.5 | 2-8 | 3,5,7,10 µm | L7 |
| NUCLEOSIL® C8 HD | high density octyl, endcapped | 13 | 2-8 | 5 µm | L7 |
| NUCLEOSIL® NH2 | aminopropyl -(CH2)3-NH2 | 3.5 | 2-8 | 5,10 µm | L8 |
| NUCLEOSIL® SA | benzenesulfonic acid, strongly acidic cation exchanger (SCX) | 6.5 | 2-8 | 5,10 µm | L9 |
| NUCLEOSIL® CN | cyanopropyl (nitrile) | 5 | 2-8 | 5,7,10 µm | L10 |
| NUCLEOSIL® Phenyl | standard phenyl, not endcapped | 8 | 2-8 | 5,7 µm | L11 |
| NUCLEOSIL® SB | quaternary ammonium groups, strongly basic anion exchanger (SAX) | 10 | 2-8 | 5,10 µm | L14 |
| NUCLEOSIL® C2 | dimethyl | 3.5 | 2-8 | 7 µm | L16 |
| NUCLEOSIL® OH | diol (dihydroxypropyl) | 5 | 2-8 | 5,7 µm | L20 |
| NUCLEOSIL® C4 | medium density butyl, endcapped | 2 | 2-8 | 5 µm | L26 |
| NUCLEOSIL® C18 Nautilus | hydrophilic octadecyl, embedded polar group, endcapped | 16 | 2-8 | 3,5 µm | L60 |
| NUCLEOSIL® Protect I | special RP phase with protective polar group, endcapped | 11 | 2-8 | 5 µm | – |
NUCLEODUR®
NUCLEODUR® ® is a fully synthetical type B silica (silica of 3rd generation) offeringhighly advanced physical properties:
- Totally spherical particle shape
- Outstanding surface microstructure
- High pressure stability
- Low metal content
Summary Of Available NUCLEODUR® Phases
| Phase | Modification | % C | pH Range | Particle Sizes [µm] | USP Classification |
| NUCLEODUR® C18 Gravity | Octadecyl, high density coating, multi-endcapping | 18 | 1–11 | 1.8, 3, 5 | L1 |
| NUCLEODUR® C18 Gravity SB | Monomeric octadecyl modification, extensive endcapping; | 13 | 1-9 | 1.8, 3, 5 | L1 |
| NUCLEODUR® C18 HTec | Octadecyl, high density coating, high capacity, multi-endcapping | 18 | 1-11 | 1.8, 3, 5, 7, 10 | L1 |
| NUCLEODUR® C18 Isis | Octadecyl, specially cross-linked modification, endcapped | 20 | 1-10 | 1.8, 3, 5 | L1 |
| NUCLEODUR® C18 Pyramid | Octadecyl with polar endcapping | 14 | 1-9 | 1.8, 3, 5 | L1 |
| NUCLEODUR® PolarTec | Octadecyl with embedded polar group | 17 | 1-9 | 3,5 | L1 L60 |
| NUCLEODUR® C18 ec | Octadecyl, medium density, endcapping | 17.5 | 1–9 | 3, 5 and larger | L1 |
| NUCLEODUR® SiOH | Unmodified NUCLEODUR® ® | – | 3, 5 and larger | L3 | |
| NUCLEODUR® C8 Gravity | Octyl, high density coating, multi-endcapping | 11 | 1-11 | 1.8, 5 | L7 |
| NUCLEODUR® C8 ec | Octyl, medium density, endcapping | 10.5 | 1-9 | 3,5 | L7 |
| NUCLEODUR® NH2/NH2-RP | Aminopropyl for NP and RP separations | 2.5 | 2–8 | 3, 5 | L8 |
| NUCLEODUR® CN/CN-RP | Cyano (nitrile) for NP and RP separations | 7 | 1-8 | 3,5 | L10 |
| NUCLEODUR® π² | Hydrophobic phase with alternative selectivity compared to classical C18 modifications | 17 | 3-10 | 5 | L11 |
| NUCLEODUR® PFP | Pentafluorophenyl-propyl with multi-endcapping | 8 | 1-9 | 3,5 | L43 |
| NUCLEODUR® Sphinx RP | Bifunctional, balanced ratio of propylphenyl and octadecyl, endcapping | 15 | 1-10 | 1.8, 3, 5 | L1 L11 |
| NUCLEODUR® HILIC | Ammonium – sulphonic acid | 7 | 2–8.5 | 1.8, 3, 5 |
NUCLEOSHELL®
- Solid core of silicon dioxide,homogeneous shell of porous silica.
- Highest efficiency compared to traditional totally porous materials.
- Pore size 90 Å; particle size 2.7 µm (core 1.7 µm); specific surface 130 m2/g.
- Lower back pressure also allows use on conventional LC systems.
- Pressure stability up to 600 bar.
| Phase | Modification | % C | pH Range | Particle Sizes [µm] | USP Classification |
| NUCLEOSHELL® RP 18 | Octadecyl modification, multi-endcapped | 7.8 for 2.7µm 6.1 for 5µm | 1–11.0 | 2.7 µm, 5 µm | L1 |
| NUCLEOSHELL® RP 18plus | polar octadecyl modification, multi-endcapped | 5.7 for 2.7µm 4.4 for 5µm | 2-9 | 2.7 µm, 5 µm | L1 |
| NUCLEOSHELL® Bluebird RP18 | Special octadecyl core-shell phase with hydrophilic endcapping | 5 | 1-8 | 2.7 µm (core 1.7 µm) | L1 |
| NUCLEOSHELL® Phenyl-Hexyl | phenyl-hexyl modification, multi-endcapped, 4.5 % C | 4.5 | 1-10 | 2.7 µm | L11 |
| NUCLEOSHELL® HILIC | Ammonium – sulfonic acid modification | 1.3 | 2–8.5 | 2.7 µm | |
| NUCLEOSHELL® PFP | Pentafluorophenyl modification, multi-endcapped | ~ 3 | 1.0–9.0 | 2.7 µm | L43 |
Columns For Special Applications
| Separation / Mechanism | Recommended Column | Specification Of The Phase | USP Classification |
| Environmental analysis | |||
| anion exchangechromatography of inorganic anions | NUCLEOGEL®Anion I | strongly basic polymer-based anion exchanger | |
| NUCLEOSIL®Anion II | strongly basic silica-based anion exchanger | ||
| RP chromatography of PAHs | NUCLEODUR® ®C18PAH, 3µm | NUCLEODUR® ®polymer-coated with C18 groups | L1 |
| NUCLEOSIL®100-5 C18PAH | NUCLEOSIL®100 polymer-coated with C18groups | L1 | |
| Enantiomer separation | |||
| based on formation of inclusion complexes | NUCLEODEX α-PM, β-PM, γ-PM and β-OH | silica-based permethylated and underivatisedcyclodextrin phases | L45 |
| based on polar and π-π interactions | NUCLEOCEL DELTA | silica-based modified cellulose phases | L40 |
| NUCLEOCEL ALPHA | silica-based modified amylosephases | L51 | |
| based on ligand exchange | NUCLEOSIL®CHIRAL-1 | covalently bonded amino acid – Cu(II) complexes | L32 |
| based on charge-transfer-, dipole-dipole interactions and others | NUCLEOSIL® CHIRAL-2 NUCLEOSIL®CHIRAL-3 | silica-based brush type phases | L36 |
| based on enantioselective bindingto chiral protein surface structures | RESOLVOSIL BSA-7 | silica-based protein phase (BSA) | |
| Biological macromolecules | |||
| anion exchangechromatography of proteins and peptide | NUCLEOSIL®4000-7 PEI | silica-based polymeric poly ethyleneimine network | |
| anion exchange chromatography of oligonucleotides and nucleic acids | NUCLEOGEN®DEAE | silica-based DEAE anion exchanger | |
| anion exchange chromatography of peptides, large proteins and oligonucleotides | NUCLEOGEL®SAX | polymer-based strongly basic anion exchanger | L23 |
| cation exchange chromatographyof proteins, peptides and carbohydrates | NUCLEOGEL®SCX | polymer-based strong cation exchanger | L22 |
| reversed phase chromatography of proteins, peptides and oligonucleotides | NUCLEOSIL®MPN | monomerically bonded alkyl chains on silica | L1L26 |
| NUCLEOSIL®PPN | polymerically bonded alkyl chains on silica | L1 | |
| NUCLEOGEL®RP 300 | polystyrene – divinylbenzene polymer | L21 | |
| reversed phasechromatography of small molecules | NUCLEOGEL®RP 100 | small pore macroporous PS-DVB polymer | L21 |
| Food analysis – Sugars | |||
| RP chromatography of mono- and oligo-saccharides | NUCLEOSIL®Carbohydrate | silica-based special amino phase | L8 |
| separation of sugars, alcohols, organic acids based on ion exclusion, ion exchange, size exclusion, ligand exchange, NP and RP effects | NUCLEOGEL® SUGAR 810 H | PS-DVB resins with sulphonic acid modification in H form | L17 |
| NUCLEOGEL® SUGAR 810 Ca | PS-DVB resins with sulphonic acid modification in Ca form | L19 | |
| separation of sugars, alcohols, organic acids based on steric exclusion, ligand exchange and partition effects | NUCLEOGEL®ION 300 OA | PS-DVB resins with sulphonic acid modification in H form | L17 |
| NUCLEOGEL® SUGAR Ca | PS-DVB resins with sulphonic acid modification in Ca form | L19 | |
| NUCLEOGEL® SUGAR Pb | PS-DVB resins with sulphonic acid modification in Pb form | L34 | |
| NUCLEOGEL® SUGAR Na | PS-DVB resins with sulphonic acid modification in Na form | L58 | |
| Gel permeation chromatography (GPC) | |||
| water-insoluble compounds | NUCLEOGEL®GPC | polystyrene – divinylbenzene polymer | |